电离辐射通过转化生长因子-β-抑制的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨电磁辐射是否可通过转化生长因子-β（TGF-β）-内皮细胞的上皮-间质反转 (EMT)来作出贡献细胞的侵袭迁移。使用存量2Gy(60)Coγ线反射源自生命体器官的6种细胞，记录下来与EMT相关的波动，这包括分别利用显微镜电子技术，蛋白质印迹方法律条文，免疫荧光电子技术，划痕测试和Transwell泽田测试来观察并侦测细胞组织形态，EMT标识，侵袭迁移能力等。采用蛋白质联免疫吸附法律条文侦测这些细胞里TGF-β蛋白程度，利用特别衍生物SB431542来审计TGF-β信号通路在电磁辐射EMT里的作用。经过存量为2Gy反射的细胞里发挥作用间叶细胞的表达，与假反射组相比其上皮标识减少，间叶细胞标识减少，同时其侵袭转移能力强化，TGF-β蛋白程度也提高。实质性找到由A549电磁辐射诱发的EMT可通过对TGF-β信号抑制发生逆转。这些最近TGF-β内皮细胞的EMT在电磁辐射诱发强化细胞侵袭转移能力里起着起着。

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